Statistical Modelling and Optimization of Extraction Condition for Protein Isolate Production from Kersting's Groundnut using Response Surface Methodology

OPTIMIZATION OF THE EXTRACTION CONDITIONS FOR PRODUCING PROTEIN ISOLATE

Keywords: Kersting’s groundnut, Optimization, Protein isolate, Central composite design (CCD), Response surface methodology (RSM)

Abstract

The study evaluated the effect of extraction conditions on the yield and protein concentration of protein isolate produced from Kersting’s groundnut, and optimized the extraction conditions using response surface methodology. The extraction conditions were solid-solvent ratio (5, 10 and 15 g/ml), temperature (30, 40 and 50 oC) and time (3, 4 and 5 hours).  The result revealed that increase in the protein isolate yield was associated with increased solid-to-solvent ratio and extraction temperature. The prediction accuracy of the RSM was supported by a non-significant lack-of-fit and R2 of 0.9999 and 0.9972 for yield and concentration of the protein isolate respectively. Hence, optimal parameters obtained were 15 g/ml solid-solvent ratio, 30 oC extraction temperature and 3 hours extraction time. A yield of 20.05% protein isolate with 70.67 mg/ml concentration was obtained based on 100 g of Kersting’s groundnut protein powder.

 

Author Biographies

Omolayo R. Osungbade, Obafemi Awolowo University, Ile-Ife, Department of Food Science and Technology

Department of Food Science and Technology

Saka O. Gbadamosi, Obafemi Awolowo University, Ile-Ife, Department of Food Science and Technology

Department of Food Science and Technology, Reader

References

Adu-Gyamfi, R., Israel, K. D. and Juliana, L., Evaluation of growth and yield potential of genotypes of Kersting’s groundnut (Macrotyloma geocarpum harms) in Northern Ghana. International Research Journal of Agricultural Science and Soil Science. 2(12):509-515, 2012.
Baş, D., and Boyacı, İ. H., Modeling and optimization I: usability of response surface methodology. Journal of Food Engineering, 78(3), 836–845, 2007.
Blaise, K., Clémence, B., Nicolas, N. Y. and Richard K., Production of Mucuna pruriens (Var. Utilis) proteins isolates using central composite design and effect of drying techniques on some properties. Chemical Science International Journal 20(1): 1-12, 2017.
Dairo, F. A. S., Aye, P. A. and Oluwasola, T. A., Some functional properties of Loofah gourd (Luffa cylindrical L., M.J. Roem) seed. Journal of Food Agriculture and Environment. 5(1): 97-101, 2007.
Duke, J. A, Okigbo, B. N., Reed, C. F., Under-exploited Legumes; Grain legume. In: Musah, A. J. (Eds.), Savanna Biodiversity Conservation Project Newsletter 1(2): 4-7, 1977.
Echendu, C. A. Obizoba, I. C. and Anyika, J. U., Effects of heat treatments on chemical composition of ground bean (Kerstingiella geocarpa harm) Pakistan Journal of Nutrition 8(12): 1877-1883, 2009.
Garba, U. and Kaur, S., Review- protein isolates: production, functional properties and application. International Research Journal of Chemistry (IRCJ). 4: 22-36, 2014.
Gbadamosi, S. O., Abiose, S. H. and Aluko, R. E., Amino acid profile, protein digestibility, thermal and functional properties of conophor nut (Tetracarpidium conophorum) defatted flour, protein concentrate and isolates. International Journal of Food Science and Technology. 47, 731-739, 2012.
Ghorbannezhad, P., Bay, A., Yolmeh, M., Yadollahi, R., and Moghadam, J. Y., Optimization of coagulation–flocculation process for medium density fiberboard (MDF) wastewater through response surface methodology. Desalination and Water Treatment, 1–16, 2016.
Kao, F., Tsou, M., Kao, H. and Chiang, W., Optimization of Enzymatic Hydrolysis Conditions for Producing Soy Protein Hydrolysate with Maximum Lipolysis-Stimulating Activity. Journal of Food and Drug Analysis, 19(2):197-201, 2011.
Kaushik, R., Saran, S., Isar, J., and Saxena, R., Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus. Journal of Molecular Catalysis B: Enzymatic, 40(3):121–126, 2006.
Kalaydzhiev, H., Brandão, T. R. S., Ivanova, P., Silva, C. L. M. and Chalova, V. I., A two-step factorial design for optimization of protein extraction from industrial rapeseed meal after ethanol-assisted reduction of antinutrients. International Food Research Journal. 26(4): 1155-1163, 2019.
Kumar, R., Sharma, R., Srivastava, T. and Saxena, D. C., Response surface methodology for optimization of protein isolate from defatted custard apple seed (Annona squamosa). International Journal of Computer Applications (0975 – 8887). International Conference on Advances in Emerging Technology (ICAET 2016).2016.
Meshkani, S. M., Mortazavi, S. A., Elhami Rad, A. H. and Beigbabaei, A., Optimization of protein extraction and evaluation of functional properties of tomato waste and seeds from tomato paste plants. Biosciences Biotechnology Research Asia. 13(4), 2387-2401, 2016.
Obasi, M. O. and Ezedinma, F. O. C., Evaluation of the growth and chemical composition of Kersting’s groundnut (Kerstingiella geocarpa Harms). Indian Journal of Agricultural Science, 61(11): 811–814, 1991.
Obasi, M. O. and Agbatse, A., Evaluation of nutritive value and some functional properties of Kersting’s groundnut seeds for optimum utilization as a food and feed resource. East Africa Agricultural For. Journal. 68: 173-181, 2003.
Oyetayo, F. L. and Ajayi, B. O., Chemical profile and zinc bioavailability on “Hausa groundnut” (Kerstingiella geocarpa). Bioscience and Biotechnology Research Asia. 3:47–50, 2005.
Sathe, S.K., Solubilisation and electrophoretic characterization of cashew nut (Anacardium occidentale) proteins. Food Chemistry 51: 319-324, 1994.
Rodrigues, I. M., Coelho, J. F., Carvalho, M. G. V., Isolation and valorisation of vegetable proteins from oilseed plants: Methods, limitations and potential. Journal of Food Engineering. 109(3):337-346, 2012.
Wang, M., Jiang L., Li, Y., Liu, Q., Wang, S. and Sui, X., Optimization of extraction process of protein isolate from mung bean. Procedia Engineering 15:5250 – 5258, 2011.
Published
2020-12-15
How to Cite
Osungbade, O. R., Gbadamosi, S. O., & Ikujenlola, A. (2020). Statistical Modelling and Optimization of Extraction Condition for Protein Isolate Production from Kersting’s Groundnut using Response Surface Methodology. Ife Journal of Technology, 27(1), 40-46. Retrieved from http://ijt.oauife.edu.ng/index.php/ijt/article/view/162