Investigation Of The Effects Of Laser Micro-Texturing On The Cellular Response Of Silicon Surfaces
Keywords:
Laser micro-texturing, microgrooves, surface modification, cellular responseAbstract
This study investigated the effects of laser micro-texturing on the interaction of silicon with cancer cells. This was with a view to improve the biocompatibility of silicon and to enhance its biomedical applications. The modification of silicon surface was carried out by the fabrication of microgrooves of different spacing on its surface using laser nano-second technique. Surface characterizations of the modified and unmodified samples were carried out using Scanning Electron Microscopy (SEM). Human osteosarcoma cells were cultured on the surfaces to study the effects of this modification process on cellular response. Optical and immunofluorescent microscopy were used to study the morphology and adsorption of cells on the different cultured samples. Micro-texturing was found to encourage the spreading of cells, with definite orientation and alignment along the groove ridges, thereby improving its biocompatibility. Increasing grooves spacing was found to enhance the adsorption and proliferation of cancer cells. This study therefore facilitates the development of substrates with improved biocompatibility for the design and fabrication of biomedical devices for cancer diagnosis and treatment.
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Walboomers, X. F., Croes, H. J. E., Ginsel, L. A and Jansen, J. A., “Growth behaviour of fibroblasts on microgrooved polystyrene.” Biomaterials. (19): 1861–1868, 1998.
Walboomers, X. F., Monaghan, A., Curtis, A. S. G. and Jansen, J. A., “Attachment of Fibroblasts on Smooth and Microgrooved Polystyrene.”, Journal of Biomedical Materials Research. (46): 212–220, 1999.
Wang, J. H. C., Grood, E.S., Florer, J and Wenstrup, R., “Alignment and Proliferation of MC3T3-E1 Osteoblasts in Microgrooved Silicone Substrate Subjected to Cyclic Stretching.” Journal of Biomechanics. (33): 729-735, 2000.
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Baseter, L.C., Fravchifer, M. Textor, M., ApGroynn, I and Richards, R. G., “Fibroblast and Osteoblast Adhesian and Morphology on Calcium phosphate surfaces.” European cells and Material. (4): 1-17, 2002.
Bhalerao, K. D., Mwenifumbo, S. C., Soboyejo, A. B. O., and Soboyejo. W. O., “Bounds in the Sensitivity of BioMEMS Devices for Cell Detection.” Biomedical Microdevices. 1 (6): 23-31, 2004.
Blazewiez, M., Czajkowska, B., Bacakova, L., Pamula, E., Blazewiez, S., “Effect of Chemical and Topographical Features of Biomaterial surface on cell Response.” European Cells and Materials. Suppl 1(7): 18, 2004.
Brunette, D. M. and Cheroudi, B., “The effect of surface topography of micro machined titanium substrata on cell behaviour in vitro and in vivo.” J. Biomed. Eng. (121): 49 – 57, 1999.
Chakraborty, R., Shahid Raza, M., Datta, S. and Saha, P., “Synthesis and characterization of nickel free titanium–hydroxyapatite composite coating over Nitinol surface through in-situ laser cladding and alloying.” Surf. Coat. Technol. (358): 539–550, 2019.
Chen, J., Ulerich, J.P., Abelev, E., Fasasi, A., Arnold, C.B. and Soboyejo, W. O., “An investigation of the initial attachment and orientation of osteoblast-like cells on laser grooved Ti–6Al–4V surfaces.” Mater. Sci. Eng. C (29): 1442–1452, 2009.
Den-Braber, G.T., de Ruijter, J.E., Smits, H.T. F., Gimsel, L.A., Von Recum, A. F and Jansen, J.A., “Effects of parallel surface microgrooves and surface energy on cell growth.” J. Biomed. Mater. Res. (29): 511 – 518, 1995.
Den-Braber, G. T., de Ruijter, J. E., Ginsel, L. A., von Recum, A. F. and Jansen, J. A., “Quantitative analysis of fibroblast morphology on microgrooved surfaces with various groove and ridge dimensions.” Biomaterials. (17): 2037–2044, 1996.
Kumari, T.V., Vazuelev, U., Kumari, A. and Menon, B., “Cell surface interactions in the study of Biocompatibility. Trends Biomater and Artif. Organ. 2 (16): 37 – 41, 2002.
Lee, I. S., Whany, C. N., KIM, H. E., Park, J. C. Song, J. H. and Kim, S. R., “Various Ca/P ratios of thin calcium phosphate films.” Materials Science and Engineering C. (22): 15-20, 2002.
Lim, J. Y. Liu, X., Volger, E. A. and Donalime, H. J., “Systematic variation in Osteoblast adhesion and phenotype with substratum surface charateristics.” J. Biomed Mater Res. (68A): 504-512, 2004.
Matsuzaka, K., Walboomers, X. F., de Ruijter, J. E. and Jansen, J. A., “The effect of poly-L-lactic acid with parallel surface micro groove on osteoblast-like cells in vitro.” Biomaterials. (20): 1293–1301, 1999.
Matsuzaka, K., Yoshinari, M., Shimono, M. and Inoue, T., “Effects of multigrooved surfaces on osteoblast-like cells in vitro: Scanning electron microscopic observation and mRNA expression of osteopontin and osteocalcin.” J. Biomed. Mater. Res. (68A): 227–234, 2003.
Milburn, C., “Effect of Surface Modification to Silicon Materials: Implications for Cell/surface Interactions and Adhesion to BioMEMS Surfaces and Microcantilever Sensors.” MSC Thesis, Department of Mechanical and Aerospace Engineering Princeton
University, Princeton, NJ., 2004.
Milburn, C., Chen, J., Cao, Y., Oparinde, G. M., Adeoye, M. O., Beye, A and Soboyejo, W. O., “Investigation of effects of Argenine- Glycine-Aspartate (RGD) and nano-scale titanium coatings on cell spreading and adhesion.” Materials science and engineering C. Biomimetic and supramolecular systems. 1(29): 306-314, 2009.
Milburn, C. and King, E., “Effects of Biocompatible Nano-Scale Coatings on Cell Spreading and Adhesion to BioMEMS Structures.” Senior Thesis, Department of Mechanical and Aerospace Engineering, Princeton University, 2004.
Mwenifumbo, S. C., “Cell surface Interactions and adhesion on Biomedical and BioMEM Surfaces; Effect of laser Micro. Texturing and Titanium Coatings.” Master’s thesis, Department of Mechanical and Aerospace Engineering, Princeton University Princeton, NJ., 2004.
Mwenifumbo, S., Li, M., Chen, J., Beye, A. and Soboyejo, W. O., “Cell/surface interactions on laser microtextured titaniumcoated silicon surfaces.” J. Mater. Sci. Mater. Med. (18): 9–23, 2007.
Nayab, S., Shinanwi, L., Hobkirk, J., Tate, T.J., Olsen, I and Jones, F.H., “Adhesion of bone cells to ion-implanted titanium.” Journal of Materials Science; Materials in Medicine. (14): 991-997, 2003.
Oyatogun G. M., Soboyejo, W. O., Afonja, A. A., Imasogie, B. I., Adewoye, O. O., Adeoye, M. O. and Esan, T. A., “Effects of Increasing Nano-Scale Coating Thickness of Titanium on The Interaction of Silicon with Cancer Cells.” Ife Journal of Technology. 1(22): 9- 14, 2013
Pfleging, W., Kumari, R., Besser, H., Scharnweber, T. and Jyotsna Dutta Majumdar, J. D., “Laser surface textured titanium alloy (Ti–6Al–4V): Part 1 – Surface characterization.” Applied Surface Science. (355): 104–111, 2015.
Price, R., Valid, M., Haberstroch, R and Webster, T., “Selective bone cell adhesion on formulations containing carbon nanofibers.” Biomaterials. 19 (24): 1827-1887, 2003.
Rao, X., Li, J., Feng, X. and Chu, C. L., “Bone-like apatite growth on controllable macroporous titanium scaffolds coated with microporous titania.” J. Mech. Behav. Biomed. (77): 225–233, 2018.
Recknor, J.B., Recknor, J.L., Sakaguehui, D. S and Mallapragada, K., “Oriented astroglial cell growth on micropatterned polystyrene substrates.’ Biomaterials. (25): 2753 – 2767, 2004.
Richards, R.G., “The effect of surface roughness on fibroblast adhesion in–vitro.” Injury. 27 (53): 38 – 43, 1996.
Smith T., “The effect of Plasma- Sprayed Coatings on the Fatigue of Titanium alloy implants." JOM. Feb 1994: 54- 56, 1994.
Soboyejo, W.O., Nemetski, B., Allameh, S., Marcantonio, N., Mercer, C and Ricci, J. L., “Interactions Between MC3T3-E1 Cells and Textured Ti6Al4V Surfaces.” Journal of Biomedical Materials Research. (62): 56-72, 2002.
Walboomers, X. F. and Jansen, J.A., “Cell and Tissue Behaviour on Micro-Grooved Surfaces.” Odontology. (89): 2-11, 2001.
Walboomers, X. F., Croes, H. J. E., Ginsel, L. A and Jansen, J. A., “Growth behaviour of fibroblasts on microgrooved polystyrene.” Biomaterials. (19): 1861–1868, 1998.
Walboomers, X. F., Monaghan, A., Curtis, A. S. G. and Jansen, J. A., “Attachment of Fibroblasts on Smooth and Microgrooved Polystyrene.”, Journal of Biomedical Materials Research. (46): 212–220, 1999.
Wang, J. H. C., Grood, E.S., Florer, J and Wenstrup, R., “Alignment and Proliferation of MC3T3-E1 Osteoblasts in Microgrooved Silicone Substrate Subjected to Cyclic Stretching.” Journal of Biomechanics. (33): 729-735, 2000.
Zafar, M.S.; Farooq, I.; Awais, M.; Najeeb, S.; Khurshid, Z.; Zohaib, S., “Bioactive surface coatings for enhancing osseointegration of dental implants.” In Biomedical, Therapeutic and Clinical Applications of Bioactive Glasses; Woodhead Publishing: Cambridge, UK, pp. 313–329, 2019.
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Published
2019-11-01
How to Cite
Oyatogun, G., Imasogie, B., Soboyejo, W., Abere, V., Oyatogun, A., & Popoola, A. (2019). Investigation Of The Effects Of Laser Micro-Texturing On The Cellular Response Of Silicon Surfaces. Ife Journal of Technology, 26(1), 74–79. Retrieved from http://ijt.oauife.edu.ng/index.php/ijt/article/view/150
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